Catalog #RP063
Recombinant human/mouse IGF-1 protein
Reactivities
Porcine, Mouse, Human, Rat, Bovine
Applications
Maintenance of pluripotency in iPSC
Cell culture media optimization and replacement of insulin in defined media
Cell culture media optimization and replacement of insulin in defined media
Product Description
Recombinant human/mouse IGF-1 protein (insulin-like growth factor 1) is a member of the insulin-like growth factor family, structurally similar to insulin, with A and B chains connected by disulfide bonds that allow low-affinity binding to the insulin receptor. IGF-1 is a primary mediator of growth hormone (GH), exerting growth-promoting effects on most cells and tissues, including skeletal muscle, cartilage, bone, liver, kidney, nerve, skin, hematopoietic cells and lung. It acts through the IGF1 receptor (IGF1R) to activate the Ras-MAPK and PI3 kinase signaling pathways, increasing cell proliferation and survival, and its activity is modulated by a group of IGF binding proteins (IGFBPs) that tend to inhibit its effects. In cell culture, recombinant IGF-1 is used to support maintenance of human embryonic stem cell (ESC) and mesenchymal stem cell (MSC) cultures, making it a valuable growth factor for stem cell culture and growth signaling research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 94%, rat - 96%, bovine - 100%, porcine - 100%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 94%, rat - 96%, bovine - 100%, porcine - 100%.
Specifications
| Reported Applications |
Maintenance of pluripotency in iPSC Cell culture media optimization and replacement of insulin in defined media |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 8 kDa |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |