Catalog #RP058
Recombinant human/mouse IGF-1 LR3 protein
Reactivities
Porcine, Mouse, Human, Rat, Bovine
Applications
Maintenance of pluripotency in iPSC
Cell culture media optimization and replacement of insulin in defined media
Cell culture media optimization and replacement of insulin in defined media
Product Description
Recombinant human/mouse IGF-1 LR3 protein (insulin-like growth factor 1 long arginine 3) is a widely used synthetic analog of IGF-1, a member of the insulin-like growth factor family whose A and B chains are connected by disulphide bonds, carrying an arginine in place of glutamic acid at position 3 (R3) and an additional 13 amino acids at its N-terminus (long, L). IGF-1 is a primary mediator of growth hormone with growth-promoting effects on skeletal muscle, cartilage, bone, liver, kidney, nerve, skin, hematopoietic and lung tissues, acting through the IGF1 receptor (IGF1R), and with low affinity the insulin receptor, to activate the Ras-MAPK and PI3 kinase pathways that increase cell proliferation and survival. Whereas native IGF-1 activity is inhibited by IGF-binding proteins (IGFBPs), IGF-1 LR3 retains binding to IGF-1 and insulin receptors but has reduced affinity for IGFBPs, giving it improved potency and metabolic stability. In cell culture, IGF-1 supports maintenance of human embryonic stem cell (ESC) and mesenchymal stem cell (MSC) cultures, making IGF-1 LR3 a potent and stable supplement for stem cell and cell culture media research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 94%, rat - 96%, bovine - 100%, porcine - 100%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 94%, rat - 96%, bovine - 100%, porcine - 100%.
Specifications
| Reported Applications |
Maintenance of pluripotency in iPSC Cell culture media optimization and replacement of insulin in defined media |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 9 kDa |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |