Catalog #RP041
Recombinant human/mouse gremlin 1 protein
Reactivities
Porcine, Mouse, Human, Rat, Bovine
Applications
Tumor organoid culture
Product Description
Recombinant human/mouse gremlin 1 protein (GREM1, isoform-1) is a member of the bone morphogenetic protein (BMP) antagonist family that binds BMP-2, BMP-4, BMP-7 and other BMP family proteins and inhibits their receptor binding. Gremlin 1 is highly expressed at the base of the small intestinal crypts, alongside the related proteins gremlin 2 and chordin-like 1, where these antagonists help maintain the stem cell population by inhibiting BMP produced by mesenchymal cells, and it is also detected in fetal brain and colon and at lower levels in adult brain, prostate, pancreas and skeletal muscle. Roles for gremlin 1 in cancer stem cell maintenance in glioblastoma have also been suggested. In cell culture, recombinant gremlin 1, like other BMP antagonists such as noggin, is used in the derivation, growth and maintenance of organoids from epithelial tissues, including intestinal, liver and pancreatic organoids, making it a key reagent for organoid and stem cell niche research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human, bovine, porcine. Species similarity: mouse - 99%, rat - 99%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human, bovine, porcine. Species similarity: mouse - 99%, rat - 99%.
Specifications
| Reported Applications | Tumor organoid culture |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 18 kDa |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in 10 mM HCl (Reconstitution Buffer A) at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |