Catalog #RP065
Recombinant human/mouse GDNF protein
Reactivities
Porcine, Mouse, Human, Rat, Bovine
Applications
Differentiation of midbrain dopaminergic neurons
Product Description
Recombinant human/mouse GDNF protein (glial cell line-derived neurotrophic factor) is a ~30 kDa cystine-knot homodimer of the GDNF family of ligands (GFL), which also includes neurturin, artemin and persephin, and signals through the RET receptor tyrosine kinase in a manner dependent on GFRα co-receptors, binding preferentially and with high affinity to GFRα1. Produced mainly by glial cells such as astrocytes, Schwann cells and satellite cells, GDNF is crucial for the development, growth and survival of neurons, particularly midbrain dopaminergic neurons, promoting their axon growth and innervation, and it also drives astrocyte differentiation, neuroprotection, kidney development and spermatogenesis. GDNF has shown therapeutic promise in Parkinson's disease, ALS, Huntington's disease and nerve and spinal cord injuries. In cell culture, GDNF is combined with BDNF, FGF-8, Shh, NT-3, IGF-1, CNTF and LIF to generate dopaminergic, glutamatergic and GABAergic neurons and astrocytes from iPSC-derived neural progenitors, and it is required with BDNF for cortical organoid maturation, making it a key reagent for neuroscience and organoid research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 93%, rat - 93%, bovine - 92%, porcine - 94%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 93%, rat - 93%, bovine - 92%, porcine - 94%.
Specifications
| Reported Applications | Differentiation of midbrain dopaminergic neurons |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 30.4 kDa (dimer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |