Catalog #RP077
Recombinant human/mouse GDF-5 protein
Reactivities
Mouse, Human, Rat
Applications
Directed chondrogenic differentiation of mesenchymal stem cells
Generation of iPSC-derived osteoblasts and chondrocytes
Mesenchymal stem cell research
Differentiation of adipose-derived stromal cells to osteogenic lineages
Generation of iPSC-derived osteoblasts and chondrocytes
Mesenchymal stem cell research
Differentiation of adipose-derived stromal cells to osteogenic lineages
Product Description
Recombinant human/mouse GDF-5 protein (growth differentiation factor 5), also known as bone morphogenetic protein 14 (BMP-14), is a member of the transforming growth factor beta (TGF-β) superfamily with a conserved signal peptide, prodomain and mature domain containing a cysteine knot formed by six conserved cysteines. GDF-5 binds type I and type II serine/threonine kinase receptors to activate Smad-dependent and Smad-independent (MAPK, PI3K and Wnt/β-catenin) pathways, regulating development, growth and survival. During embryonic development and tissue homeostasis, GDF-5 promotes formation and maintenance of bones and joints and regulates osteoblast and chondrocyte proliferation and differentiation. Loss-of-function GDF-5 mutations are linked to Hunter-Thompson syndrome, brachydactyly type C and DuPan syndrome, and polymorphisms to increased osteoarthritis risk. In cell culture, recombinant GDF-5 is used for differentiation and maintenance of induced pluripotent, embryonic and bone marrow-derived mesenchymal stem cells, promotes osteoblast and chondrocyte differentiation with BMP-4, and stimulates osteogenic differentiation of adipose-derived stromal cells with TGF-β1, making it a key reagent for bone and joint regeneration research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 99%, rat - 98%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 99%, rat - 98%.
Specifications
| Reported Applications |
Directed chondrogenic differentiation of mesenchymal stem cells Generation of iPSC-derived osteoblasts and chondrocytes Mesenchymal stem cell research Differentiation of adipose-derived stromal cells to osteogenic lineages |
|---|---|
| Formulation | Lyophilized, white powder |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 27 kDa (monomer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in 10 mM HCl (Reconstitution Buffer A) at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |