Catalog #RP055
Recombinant human/mouse follistatin-resistant activin A (FRACTA) protein
Reactivities
Porcine, Mouse, Human, Rat, Bovine
Applications
Induced pluripotent and embryonic stem cell differentiation and maintenance
Product Description
Recombinant human/mouse follistatin-resistant activin A (FRACTA) protein is an engineered form of activin A, a member of the TGF-β superfamily and a disulfide-linked homodimer of inhibin βA chains, synthesized as a precursor with a signal peptide, a 250–350-residue pro-domain and a highly conserved mature domain. Activin A is involved in stem cell differentiation and maintenance, embryogenesis, reproductive system development, wound healing and immune regulation, and its signaling is modulated by inhibins and by follistatin, a glycoprotein whose production is stimulated by activin A in a feedback loop and which neutralizes TGF-β superfamily members by shielding their type I and type II receptor binding sites. Recombinant activin A is widely used to maintain pluripotency in human induced pluripotent and embryonic stem cell lines and to induce differentiation into endoderm and other cell fates. Developed in the lab of Marko Hyvönen at the University of Cambridge, FRACTA is engineered to be unimpeded by follistatin while retaining its ability to bind type I and II receptors, making it a valuable tool for stem cell culture and differentiation where follistatin feedback limits activin A activity.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 98%, rat - 98%, bovine - 98%, porcine - 98%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 98%, rat - 98%, bovine - 98%, porcine - 98%.
Specifications
| Reported Applications | Induced pluripotent and embryonic stem cell differentiation and maintenance |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 26 kDa (dimer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in 10 mM HCl (Reconstitution Buffer A) at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |