Catalog #RP067
Recombinant human/mouse FGF2-G3 (154 aa) protein
Reactivities
Porcine, Mouse, Human, Rat, Bovine
Applications
Expansion of induced pluripotent, embryonic and mesenchymal stem cells
Product Description
Recombinant human/mouse FGF2-G3 (154 aa) protein, also known as thermostable FGF-2 or FGF2-STAB®, is an engineered form of basic fibroblast growth factor (bFGF), the essential growth factor for maintaining human embryonic stem cell (hESC) and induced pluripotent stem cell (iPSC) pluripotency in feeder-free, chemically defined media such as mTeSR, StemPro and E8. Wild-type FGF-2 is prone to proteolytic degradation and aggregation, with a half-life in culture media of less than 10 hours, driving frequent media changes and heterogeneity during proliferation and differentiation. Engineered using computer-assisted protein design, FGF2-G3 carries nine stabilizing amino acid substitutions designed to preserve the FGFR1 and FGFR2 binding sites, and it shows no loss of biological activity after more than 7 days in conditioned media at 37 °C, compared with less than 50% activity for wild-type FGF-2 after 10 hours, while maintaining Oct-4 and nanog expression with equivalent efficacy. FGF2-G3 is the thermostable FGF-2 used in B8 iPSC maintenance media, enabling lower media cost and weekend-free culture, and this His-tag-free protein supports translation and scale-up, making it a key reagent for stem cell culture, regenerative medicine and cultured meat applications.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human/mouse. Species similarity: rat - 100%, bovine - 100%, porcine - 100%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human/mouse. Species similarity: rat - 100%, bovine - 100%, porcine - 100%.
Specifications
| Reported Applications | Expansion of induced pluripotent, embryonic and mesenchymal stem cells |
|---|---|
| Formulation | Lyophilized, white powder |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 17 kDa |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |