Catalog #RP066
Recombinant human/mouse FGF2-G3 (145 aa) protein
Reactivities
Porcine, Mouse, Human, Rat, Bovine
Applications
Induced pluripotent stem cell culture and maintenance
Product Description
Recombinant human/mouse FGF2-G3 (145 aa) protein, also known as FGF2-STAB®, is a thermostable engineered form of FGF-2 (basic FGF, bFGF), the essential growth factor for maintaining human embryonic stem cell (hESC) and induced pluripotent stem cell (iPSC) pluripotency in feeder-free, chemically defined media such as mTeSR, StemPro and E8. Wild-type FGF-2 is inherently unstable, prone to proteolytic degradation and aggregation and has a half-life of less than 10 h in culture, contributing to frequent media changes and poor homogeneity during expansion and differentiation. Using computer-assisted engineering, Dvorak and colleagues at Masaryk University identified nine stabilizing substitutions that preserve the FGFR1 and FGFR2 binding sites; whereas wild-type FGF-2 loses over 50% of its activity after 10 h in conditioned media, FGF2-G3 shows no loss of activity after more than 7 days at 37 °C, while maintaining hESC pluripotency and Oct-4 and nanog expression with equivalent efficacy. FGF2-G3 is used in B8 medium, the low-cost, weekend-free iPSC protocol published by Paul Burridge and colleagues, and in emerging cultured meat applications, and this His tag-free protein avoids tag-related issues in clinical translation and scale-up, making it a gold-standard reagent for stem cell culture media.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human/mouse. Species similarity: rat - 100%, bovine - 100%, porcine - 100%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human/mouse. Species similarity: rat - 100%, bovine - 100%, porcine - 100%.
Specifications
| Reported Applications | Induced pluripotent stem cell culture and maintenance |
|---|---|
| Formulation | Lyophilized, white powder |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 16 kDa |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |