Catalog #RP016
Recombinant human/mouse FGF-8b protein
Product Description
Recombinant human FGF-8b protein is a 22.5 kDa, 194-amino-acid heparin-binding isoform of FGF-8 within the fibroblast growth factor (FGF) family, originally identified as a growth factor for androgen-dependent mouse mammary carcinoma. Of the four human FGF-8 isoforms (a, b, e and f), FGF-8b shows the strongest receptor affinity and oncogenic capacity, with human and murine FGF-8a and FGF-8b sharing 100% homology. Containing a conserved heparin-binding domain essential for bioactivity, FGF-8b binds FGF receptors to activate the Ras/MAPK pathway and drives cell proliferation, differentiation, survival and apoptosis. It is a key regulator of embryonic development and epithelial-mesenchymal transitions, patterning the brain, eye, ear, limb and heart, and it transforms the midbrain into cerebellum in transgenic models. In cell culture, FGF-8b promotes neurite outgrowth and neural regeneration and, combined with Shh and transcription factors, is used to generate dopaminergic neurons and iPSC-derived ventral midbrain organoids for 3D neuronal research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Human and murine FGF-8a and FGF-8b show 100% homology.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Human and murine FGF-8a and FGF-8b show 100% homology.
Specifications
| Formulation | Lyophilized, white powder |
|---|---|
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 22.5 kDa |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |