Catalog #RP034
Recombinant human/mouse FGF-4 protein
Reactivities
Porcine, Mouse, Human, Bovine
Applications
Hindgut organoids from iPSC-derived human posterior gut endoderm cells
Neural stem cell proliferation and neuronal differentiation
Neural stem cell proliferation and neuronal differentiation
Product Description
Recombinant human/mouse FGF-4 protein (fibroblast growth factor 4) is a member of the FGF superfamily with a physiological role in regulating the proliferation and differentiation of embryonic stem cells and tissue stem cells. Recombinant FGF-4 promotes neural stem cell proliferation and neuronal differentiation in the postnatal brain, increases the proliferation rate of human adult bone marrow-derived mesenchymal stem cells, and supports the maintenance, proliferation and self-renewal of human embryonic stem cells. Acting in synergy with WNT signaling, FGF-4 drives the formation of hindgut organoids from iPSC-derived human posterior gut endoderm, and it is used to mimic embryonic intestinal development during the directed differentiation of pluripotent stem cells into intestinal organoids, making it a key reagent for stem cell, neural and intestinal organoid research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 91%, rat - 80%, bovine - 90%, porcine - 91%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 91%, rat - 80%, bovine - 90%, porcine - 91%.
Specifications
| Reported Applications |
Hindgut organoids from iPSC-derived human posterior gut endoderm cells Neural stem cell proliferation and neuronal differentiation |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 14 kDa |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |