Catalog #RP050
Recombinant human/mouse FGF-2 (154 aa) protein
Reactivities
Porcine, Mouse, Human, Rat, Bovine
Applications
Induced pluripotent and embryonic stem cell differentiation and maintenance
Product Description
Recombinant human/mouse FGF-2 (154 aa) protein, also known as basic fibroblast growth factor (bFGF), is a member of the fibroblast growth factor (FGF) family, which comprises 18–22 human members in 6–7 subfamilies and regulates cell proliferation, survival, migration and differentiation. FGF-2 is one of the FGFs that binds and signals through all four transmembrane tyrosine kinase receptors (FGFR1–4), which recruit SH2- or PTB-domain signaling proteins to activate the RAS/MAP kinase, PI3 kinase/Akt and PLCγ pathways. FGFs play critical roles in embryonic development and, in adults, modulate tissue maintenance, wound healing and angiogenesis. FGF-2 is an essential growth factor for maintaining pluripotency in human embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs) and mesenchymal stem cells (MSCs), preserving stemness through PI3K/Akt, ERK1/2, JAK/STAT and PLCγ signaling and activin A activation, and in feeder systems it stimulates production of essential growth factors and cytokines from the feeder layer. Together with TGF-β1, FGF-2 is required for feeder-free iPSC maintenance, and this high-quality, bioactive and animal origin-free FGF-2 is a key reagent for stem cell culture.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 94%, rat - 97%, bovine - 99%, porcine - 99%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 94%, rat - 97%, bovine - 99%, porcine - 99%.
Specifications
| Reported Applications | Induced pluripotent and embryonic stem cell differentiation and maintenance |
|---|---|
| Formulation | Lyophilized, white powder |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 17 kDa |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |