Catalog #RP032
Recombinant human/mouse activin A protein
Reactivities
Porcine, Mouse, Human, Rat, Bovine
Applications
Induced pluripotent and embryonic stem cell differentiation and maintenance
iPSC-derived mesoderm differentiation
Differentiation of iPSC into endoderm
Chemically defined media for organoid culture
iPSC-derived mesoderm differentiation
Differentiation of iPSC into endoderm
Chemically defined media for organoid culture
Product Description
Recombinant human/mouse activin A protein is a disulfide-linked homodimer of inhibin βA chains and a member of the transforming growth factor beta (TGF-β) superfamily, first identified for its ability to stimulate follicle-stimulating hormone (FSH) release from the pituitary and synthesized as a precursor with a signal peptide, a 250–350-residue pro-domain and a highly conserved mature domain. Activin A binds type I (ALK4 or ALK7) and type II (ActRIIA or ActRIIB) receptors to drive SMAD-dependent transcription, and its activity is tightly regulated by follistatin and inhibins in a feedback loop. It regulates embryonic development, cell proliferation and differentiation, mesoderm, neural and reproductive system patterning, immune responses and wound healing, and impaired activin A signaling is associated with cancer, inflammation and fibrosis. In culture, activin A is used with FGF-2 to maintain pluripotency of human iPSCs and ESCs, directs differentiation into definitive endoderm for pancreatic and liver lineages, promotes neural precursors and astrocytic differentiation with CNTF and GDNF, supports mesodermal differentiation into muscle, bone and blood cells, and is widely used for organoid development and maintenance, making it a key reagent for stem cell and organoid research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 100%, rat - 100%, bovine - 100%, porcine - 100%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 100%, rat - 100%, bovine - 100%, porcine - 100%.
Specifications
| Reported Applications |
Induced pluripotent and embryonic stem cell differentiation and maintenance iPSC-derived mesoderm differentiation Differentiation of iPSC into endoderm Chemically defined media for organoid culture |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 26 kDa (dimer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in 10 mM HCl (Reconstitution Buffer A) at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |