Catalog #RP079
Recombinant human M-CSF protein
Reactivities
Human
Applications
Differentiation of iPSC-derived macrophages
Culture of bone marrow-derived macrophages
Maintenance of macrophages
Polarization of anti-inflammatory or M2 macrophage
Differentiation of alveolar macrophages to study fibrotic disorders
Osteoclast culture
Culture of bone marrow-derived macrophages
Maintenance of macrophages
Polarization of anti-inflammatory or M2 macrophage
Differentiation of alveolar macrophages to study fibrotic disorders
Osteoclast culture
Product Description
Recombinant human M-CSF protein (macrophage colony-stimulating factor), also known as colony-stimulating factor-1 (CSF-1), is a 36 kDa secreted hematopoietic growth factor with a dimeric structure of two covalently linked four-helix bundles, expressed as three major isoforms that share a 149-amino-acid N-terminal region required for activity. M-CSF binds the receptor tyrosine kinase CSF-1R on monocytes, macrophages, microglia, osteoclasts and dendritic cells, activating PI3K-AKT and AMPK signaling to regulate the survival, proliferation, renewal and differentiation of the monocyte-macrophage lineage and other myeloid cells. Elevated M-CSF is associated with inflammatory and tumor-associated immunosuppressive conditions, making the M-CSF/CSF-1R axis a target of interest in glioma and stroke. In culture, recombinant M-CSF is used to differentiate hiPSC-derived macrophages, to maintain monocytes in hematopoietic stem cell culture, to direct myeloid cells with IL-3 toward phagocytic macrophages, to induce PU.1-driven myeloid fate and an anti-inflammatory M2 phenotype, and to generate monocyte-derived macrophages modeling alveolar macrophages in fibrosis, making it a key reagent for macrophage and myeloid biology research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 74%, rat - 54%, bovine - 66%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 74%, rat - 54%, bovine - 66%.
Specifications
| Reported Applications |
Differentiation of iPSC-derived macrophages Culture of bone marrow-derived macrophages Maintenance of macrophages Polarization of anti-inflammatory or M2 macrophage Differentiation of alveolar macrophages to study fibrotic disorders Osteoclast culture |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 36 kDa (dimer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in 10 mM HCl (Reconstitution Buffer A) at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |