Catalog #RP100
Recombinant human IL-8 protein
Reactivities
Human
Applications
Differentiation into monocytic lineages
Chemotaxis experiments
Differentiation of osteoclasts
Chemotaxis experiments
Differentiation of osteoclasts
Product Description
Recombinant human IL-8 protein (interleukin-8 / CXCL8) is a C-X-C motif chemokine, originally identified as monocyte-derived neutrophil chemotactic factor (MDNCF) for its chemotactic activity on neutrophils, translated as a 99-amino-acid precursor and processed to the 72-amino-acid biologically active form. IL-8 signals through two G-protein coupled receptors, CXCR1 and CXCR2, activating Akt/PKB, MAPK and PKC pathways that regulate cell adhesion and migration. An inflammatory cytokine produced by fibroblasts, lung epithelial and endothelial cells, keratinocytes, T cells, neutrophils, mast cells, monocytes and macrophages, IL-8 also contributes to tumor growth and vascularization, and in triple negative breast cancer its expression is closely related to prognosis and is being investigated as a potential therapeutic target. IL-8 further acts as a growth factor for human hematopoietic progenitors, promoting growth and differentiation of monocytic lineages including megakaryocytes, monocytes and macrophages, and recombinant IL-8 stimulates differentiation of human peripheral blood mononuclear cells into osteoclasts, making it a key reagent for inflammation, cancer and hematopoiesis research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 80%, bovine - 76%, porcine - 76%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 80%, bovine - 76%, porcine - 76%.
Specifications
| Reported Applications |
Differentiation into monocytic lineages Chemotaxis experiments Differentiation of osteoclasts |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 8.9 kDa (monomer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in 10 mM HCl (Reconstitution Buffer A) at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |