Catalog #RP029
Recombinant human IL-7 protein
Product Description
Recombinant human IL-7 protein (interleukin-7) is a ~17.5 kDa non-glycosylated four-alpha-helix cytokine that binds the IL-7 receptor, a heterodimer of the IL-7Rα chain (CD127) and the common gamma chain (γc, CD132), activating JAK-STAT signaling and STAT5 phosphorylation to promote T-cell survival, proliferation and differentiation. IL-7 is essential for T-cell homeostasis, supporting thymocyte development and the maintenance of naive and memory T cells for effective immune surveillance, and it also contributes to B-cell and, to a lesser extent, natural killer (NK) cell development. Clinically, IL-7 is studied in cancer immunotherapy to enhance T-cell recovery after chemotherapy or radiation and alongside checkpoint inhibitors, in HIV and chronic infections to restore CD4+ T-cell counts, in bone marrow transplantation to accelerate T-cell recovery and mitigate graft-versus-host disease, in autoimmune diseases such as multiple sclerosis and rheumatoid arthritis, and as a vaccine adjuvant and regenerative-medicine tool, making recombinant IL-7 a valuable reagent for immunology and immunotherapy research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: human. Species similarity: mouse - 56%, rat - 57%, bovine - 71%, porcine - 69%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: human. Species similarity: mouse - 56%, rat - 57%, bovine - 71%, porcine - 69%.
Specifications
| Formulation | Lyophilized, white powder |
|---|---|
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 17.5 kDa (monomer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |