Catalog #RP088
Recombinant human IL-34 protein
Reactivities
Human
Applications
Polarization of anti-inflammatory or M2 macrophage
Generation of iPSC-derived microglia and Langerhans cells
Osteoclast culture to study osteoporosis and bone disorders
Generation of iPSC-derived microglia and Langerhans cells
Osteoclast culture to study osteoporosis and bone disorders
Product Description
Recombinant human IL-34 protein (interleukin-34) is a dimeric four-helix bundle cytokine, natively 241 amino acids and 39 kDa, that regulates the differentiation, survival and activation of monocytes, macrophages and osteoclasts. Produced mainly by keratinocytes and neurons, as well as fibroblasts, endothelial cells and certain immune cells, IL-34 was identified in 2008 as a ligand for colony-stimulating factor 1 receptor (CSF-1R), which it shares with M-CSF (CSF-1) despite distinct expression patterns. IL-34 is key to the development and maintenance of tissue-resident macrophages such as Langerhans cells and microglia and induces differentiation of monocytes into immunosuppressive M2 macrophages. Its dysregulation is implicated in rheumatoid arthritis, multiple sclerosis, Alzheimer's and Parkinson's disease and cancers such as colorectal cancer and hepatocarcinoma, where it recruits tumor-associated macrophages. In vitro, IL-34 is used to differentiate, activate and polarize bone marrow-derived monocytes into macrophages, to maintain and differentiate human iPSCs into microglia, to promote osteoclast differentiation for bone resorption studies and to study cancer cell proliferation and migration, making it a key reagent for immunology, neuroscience and bone research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 71%, rat - 72%, bovine - 78%, porcine - 75%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 71%, rat - 72%, bovine - 78%, porcine - 75%.
Specifications
| Reported Applications |
Polarization of anti-inflammatory or M2 macrophage Generation of iPSC-derived microglia and Langerhans cells Osteoclast culture to study osteoporosis and bone disorders |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 25 kDa (monomer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |