Catalog #RP107
Recombinant human IL-21 protein
Reactivities
Human
Applications
Stimulation of activated T cell proliferation
Development, survival, and function of T cells
Activation of T-cells and natural killer (NK) cells
Differentiation of B cells into plasma cells
Stimulation of activated B cell proliferation
Development and formation of B cells
Development, survival, and function of T cells
Activation of T-cells and natural killer (NK) cells
Differentiation of B cells into plasma cells
Stimulation of activated B cell proliferation
Development and formation of B cells
Product Description
Recombinant human IL-21 protein (interleukin-21) is a potent immunoregulatory cytokine produced by activated natural killer T (NKT) cells, T helper 17 (Th17) cells and T follicular helper (TFH) cells, with expression induced by T cell receptor signaling and by cytokines such as IL-1β, IL-6 and IL-27. IL-21 signals through a heterodimeric receptor composed of the IL-21-specific receptor (IL-21R) and the common γ-chain (γC), which is shared with IL-2, IL-4, IL-7, IL-9 and IL-15, activating the JAK-STAT, MAPK and PI3K pathways. It stimulates the activation, proliferation and survival of CD8+ T cells and NKT cells, promotes Th17 polarization and, together with TGF-β1, drives the differentiation of human naive CD4+ T cells into Th17 cells. IL-21 is also critical for B cell activation, proliferation, differentiation, apoptosis, antibody production and differentiation into plasma cells, and it is required for dendritic cell migration to draining lymph nodes. These broad immunostimulatory effects have made IL-21 a therapeutic target of interest, including for increasing the susceptibility of myeloid leukemia cells to CAR T cell therapy and improving survival, making recombinant IL-21 a key reagent for immunology and immunotherapy research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: human.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: human.
Specifications
| Reported Applications |
Stimulation of activated T cell proliferation Development, survival, and function of T cells Activation of T-cells and natural killer (NK) cells Differentiation of B cells into plasma cells Stimulation of activated B cell proliferation Development and formation of B cells |
|---|---|
| Formulation | Lyophilized, white powder |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 15.3 kDa (monomer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in 10 mM HCl (Reconstitution Buffer A) at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |