Catalog #RP092
Recombinant human IL-18 protein
Reactivities
Human
Applications
Drug discovery in inflammation
Development and formation of NK cells
Stimulation of activated T cell proliferation
Hematopoietic stem cell regulation
Autoimmune disease research
Cancer immunotherapy
Development and formation of NK cells
Stimulation of activated T cell proliferation
Hematopoietic stem cell regulation
Autoimmune disease research
Cancer immunotherapy
Product Description
Recombinant human IL-18 protein (interleukin-18) is a pro-inflammatory cytokine of the IL-1 family, produced by macrophages and dendritic cells as an inactive precursor that is activated by caspase-1 cleavage into a ~18 kDa mature protein with a β-trefoil fold. IL-18 binds its receptor (IL-18R) to trigger NF-κB and MAPK signaling, stimulates IFN-γ production by NK cells and CD4+ T-cells to strengthen antiviral and antibacterial defense, works with IL-12 to drive Th1 differentiation, induces TNF-α and promotes immune cell migration by increasing adhesion molecule expression. Excess IL-18 contributes to inflammation in rheumatoid arthritis and inflammatory bowel disease, while deficiency is linked to obesity and type 2 diabetes through effects on insulin sensitivity and lipid metabolism. IL-18 is a therapeutic target in autoimmunity, is explored in cancer immunotherapy to enhance NK and T-cell responses, and is studied as a vaccine adjuvant and in HIV and hepatitis C. It also induces quiescence in hematopoietic stem cells, and its modulation may improve allogeneic stem cell transplantation outcomes, making recombinant IL-18 a key reagent for immunology and stem cell research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 66%, rat - 64%, bovine - 78%, porcine - 75%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 66%, rat - 64%, bovine - 78%, porcine - 75%.
Specifications
| Reported Applications |
Drug discovery in inflammation Development and formation of NK cells Stimulation of activated T cell proliferation Hematopoietic stem cell regulation Autoimmune disease research Cancer immunotherapy |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 18 kDa (monomer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |