Catalog #RP039
Recombinant human HGF NK1 protein
Reactivities
Porcine, Human, Rat, Bovine
Applications
Differentiation of iPSCs to hepatocyte-like cells
Chemically defined media for organoid culture
Airway, mammary, liver hepatocyte, and urothelium organoids
Chemically defined media for organoid culture
Airway, mammary, liver hepatocyte, and urothelium organoids
Product Description
Recombinant human HGF NK1 protein (hepatocyte growth factor, also called scatter factor) is the smallest naturally occurring splice variant of HGF, comprising the N-terminal hairpin loop domain involved in c-Met binding and the first kringle domain. Full-length HGF is secreted as a single inactive polypeptide that is cleaved by serine proteases into a 62 kDa heavy chain and a 32–36 kDa light chain, joined by a disulfide bond to form the active αβ heterodimer, and it signals through the receptor tyrosine kinase c-Met to activate PI3K/AKT, FAK, JNK and ERK1/2 signaling. HGF is a multifunctional mitogen for mesenchymal, endothelial and epithelial cells that stimulates liver, kidney and lung development and, in adults, regulates cell motility, survival, angiogenesis and tissue regeneration, particularly liver regeneration, while dysregulated HGF/c-Met signaling contributes to tumor growth, invasion and metastasis. In cell culture, HGF supports the growth and maintenance of hepatocytes and endothelial cells, promotes osteogenic, chondrogenic and adipogenic differentiation of mesenchymal cells, and is used to study cell cycle, motility, invasion and wound healing, making HGF NK1 a valuable reagent for regenerative medicine and cancer research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 89%, rat - 90%, bovine - 93%, porcine - 95%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 89%, rat - 90%, bovine - 93%, porcine - 95%.
Specifications
| Reported Applications |
Differentiation of iPSCs to hepatocyte-like cells Chemically defined media for organoid culture Airway, mammary, liver hepatocyte, and urothelium organoids |
|---|---|
| Formulation | Lyophilized, white powder |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 20 kDa |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in 10 mM HCl (Reconstitution Buffer A) at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |