Catalog #RP105
Recombinant human GDF-11 (BMP-11) protein
Reactivities
Human
Applications
Neurogenesis and neural differentiation
Wound healing and tissue repair
Wound healing and tissue repair
Product Description
Recombinant human GDF-11 (BMP-11) protein (growth differentiation factor 11) is a cystine-knot cytokine of the BMP/GDF family within the TGF-β superfamily, closely homologous to GDF-8/myostatin and a key regulator of cell growth and differentiation during muscular and neural development. Like GDF-8, GDF-11 binds activin type 2 receptors and recruits ALK4 or ALK5 to activate Smad2/3 signaling, but it can also recruit ALK7 or ALK1 to activate Smad1/5/9, which may underlie its distinct signaling and developmental responses. Circulating GDF-11 levels decline with age, highlighting it as a potential therapeutic in age-related diseases, and recombinant GDF-11 exerts pro-myogenic effects in skeletal muscle, anti-hypertrophic effects in the heart and beneficial effects on neurogenesis and neuronal function relevant to stroke. In culture, it increases proliferation of aged satellite cells, an effect not seen with GDF-8 or TGF-β1, dose-dependently decreases adipogenesis in mesenchymal stem cells via ALK4/5-dependent Smad2/3 signaling, and reduces osteoblast and chondrocyte differentiation, making it a key reagent for aging, muscle, bone and stem cell research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: human.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: human.
Specifications
| Reported Applications |
Neurogenesis and neural differentiation Wound healing and tissue repair |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 25 kDa (dimer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in 10 mM HCl (Reconstitution Buffer A) at >50 μg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |