Catalog #RP007
Recombinant human FGF-2 (145 aa) protein
Reactivities
Mouse, Human, Rat, Bovine
Applications
Cell culture (differentiation, maintenance/expansion)
Organoid / 3D culture
Functional bioassay
Organoid / 3D culture
Functional bioassay
Product Description
Recombinant human FGF-2 (145 aa) protein, also known as basic fibroblast growth factor (bFGF), is a member of the fibroblast growth factor (FGF) family that regulates cell growth, survival, proliferation, migration and differentiation. FGF-2 is unique in binding and signaling through all four FGF receptors (FGFR1–4), activating the RAS/MAP kinase, PI3K/Akt, JAK/STAT and PLCγ pathways. It is an essential growth factor for maintaining pluripotency in human embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs) and mesenchymal stem cells (MSCs), preserving stemness via PI3K/Akt, ERK1/2, JAK/STAT and PLCγ signaling and activin A activation, and it stimulates essential growth factor and cytokine production from feeder layers. Together with TGF-β1, FGF-2 is required for feeder-free iPSC maintenance; because native FGF-2 has poor thermostability in culture, this FGF2-G3 variant is engineered for high thermostability, enabling weekend-free iPSC culture without daily media changes.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: human. Species similarity: mouse - 97%, rat - 97%, bovine - 99%, porcine - 99%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: human. Species similarity: mouse - 97%, rat - 97%, bovine - 99%, porcine - 99%.
Specifications
| Reported Applications |
Cell culture (differentiation, maintenance/expansion) Organoid / 3D culture Functional bioassay |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 17 kDa |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |