Catalog #RP091
Recombinant human EPO protein
Reactivities
Human
Applications
Maintenance and differentiation of hematopoietic stem cells
Proliferation and differentiation of erythrocytes, leukocytes and platelets
Maintenance of cardiac progenitor cells
Proliferation and differentiation of erythrocytes, leukocytes and platelets
Maintenance of cardiac progenitor cells
Product Description
Recombinant human EPO protein (erythropoietin) is a 30.4 kDa glycoprotein hormone and essential cytokine, produced mainly by the kidneys, that controls erythropoiesis, the production of red blood cells. EPO binds the EPO receptor (EPOR), a single-pass member of the cytokine receptor superfamily whose ligand-induced dimerization leads to phosphorylation and activation of Janus kinase 2 (JAK-2) and downstream STAT-5, PI-3K/AKT and SHC/MAPK signaling. Hypoxia stimulates EPO expression, promoting viability, proliferation and terminal differentiation of erythroid precursors to increase red blood cell mass, and basal EPO levels maintain circulating red blood cell and hemoglobin levels, so that loss of EPO through neutralizing antibodies or chronic kidney disease leads to anemia. In cell culture, recombinant EPO is combined with SCF, TPO, Flt3L, IL-7 and IL-15 to differentiate hematopoietic progenitors into eight lineages, including erythrocytes, platelets, macrophages, granulocytes, dendritic cells, NK, B and T cells, and is used in cardiac progenitor cell culture, with human EPO also suitable for mouse hematopoietic and cardiac progenitors, making it a key reagent for hematopoiesis research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 80%, rat - 83%, bovine - 83%, porcine - 82%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 80%, rat - 83%, bovine - 83%, porcine - 82%.
Specifications
| Reported Applications |
Maintenance and differentiation of hematopoietic stem cells Proliferation and differentiation of erythrocytes, leukocytes and platelets Maintenance of cardiac progenitor cells |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 18.4 kDa (monomer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |