Catalog #RP075
Recombinant human DKK-1 protein
Reactivities
Human, Bovine
Applications
Differentiation of iPSC derived neural progenitors to neurons
Osteogenic differentiation
Maintenance of pluripotency in iPSC
Mesenchymal stem cell research
Expansion of induced pluripotent, embryonic and mesenchymal stem cells
Osteogenic differentiation
Maintenance of pluripotency in iPSC
Mesenchymal stem cell research
Expansion of induced pluripotent, embryonic and mesenchymal stem cells
Product Description
Recombinant human DKK-1 protein (Dickkopf-related protein 1) is a secreted member of the DKK family, alongside DKK-2, DKK-3 and DKK-4, originally identified as a Xenopus head-forming molecule and now recognized as a potent antagonist of the canonical Wnt/β-catenin pathway. Structurally, DKK-1 contains an N-terminal signal peptide, a conserved cysteine-rich domain and a linker to a C-terminal region, and it forms inhibitory complexes with the LRP5/6 co-receptors and Kremen, triggering endocytosis and removal of LRP5/6 from the cell surface. DKK-1 inhibition of Wnt signaling is indispensable for anterior development, as mice lacking DKK-1 fail to form a head, and dysregulated Wnt signaling links DKK-1 to cancers such as esophageal squamous cell carcinoma and hormone-resistant breast cancer. In cell culture, DKK-1 regulates the balance between self-renewal and differentiation in embryonic stem cells, directs iPSC differentiation toward neural lineages, guides osteogenic differentiation of mesenchymal stem cells for bone tissue engineering, and supports in vitro modeling of Wnt-driven disease, making it a key reagent for stem cell and developmental biology research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 86%, rat - 86%, bovine - 93%, porcine - 33%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 86%, rat - 86%, bovine - 93%, porcine - 33%.
Specifications
| Reported Applications |
Differentiation of iPSC derived neural progenitors to neurons Osteogenic differentiation Maintenance of pluripotency in iPSC Mesenchymal stem cell research Expansion of induced pluripotent, embryonic and mesenchymal stem cells |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 25.7 kDa (monomer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |