Catalog #RP003
Recombinant human BMP-2 protein
Reactivities
Mouse, Human, Rat, Bovine
Product Description
Recombinant human BMP-2 protein (bone morphogenetic protein 2) is a member of the BMP subgroup of the transforming growth factor beta (TGF-β) superfamily and a potent regulator of embryonic patterning, organogenesis, limb bud formation, and bone and cartilage growth. Synthesized as a preproprotein and proteolytically processed to a 114-residue mature domain, BMP-2 forms active disulfide-linked homodimers as well as heterodimers with BMP-7. Mature human BMP-2 shares 100% amino acid sequence identity with mouse and rat BMP-2 and 85% identity with BMP-4, but less than 51% with other BMPs. As a strong differentiation factor, recombinant BMP-2 directs human pluripotent stem cells toward extra-embryonic endoderm, mesenchymal and neural lineages, and chondrocytes, and induces bone and cartilage formation in vitro and chondrogenesis in adult mesenchymal stem cells, making it a key growth factor for stem cell differentiation and bone/cartilage tissue-engineering research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: human. Species similarity: mouse - 100%, rat - 100%.
It also shares 85% identity with BMP-4, but <51% with other BMPs.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: human. Species similarity: mouse - 100%, rat - 100%.
It also shares 85% identity with BMP-4, but <51% with other BMPs.
Specifications
| Recommended Dilution Buffer | InVivoPure Reconstitution Buffer A |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 26 kDa (dimer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in 10 mM HCl (Reconstitution solution A) at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |