Catalog #RP104
Recombinant human betacellulin
Reactivities
Human, Bovine
Applications
Cellular proliferation, migration and survival
Stimulation of angiogenesis and vascular network development
Neurogenesis and neural differentiation
Differentiation of retinal progenitor cells
Stimulation of angiogenesis and vascular network development
Neurogenesis and neural differentiation
Differentiation of retinal progenitor cells
Product Description
Recombinant human betacellulin (BTC) is a member of the epidermal growth factor (EGF) family, originally identified as a proliferation factor in conditioned media from a mouse pancreatic tumor cell line, encoded as a 178-amino-acid membrane-anchored precursor that is cleaved to an 80-amino-acid mature form. Betacellulin binds the EGF receptor (EGFR) and other ERBB receptors, activating intracellular pathways including p38 MAPK, PI3K/AKT and JNK. As a pancreatic growth factor, it stimulates neogenesis and proliferation of pancreatic β-cells and pancreatic tumor cell lines and can convert selected non-β-cells, including mesenchymal stem cells (MSCs), into insulin-producing cells. Betacellulin may also contribute to angiogenesis, being detected in models of retinal neovascularization and inducing retinal vascular permeability and edema. It plays a regenerative role in the neural stem cell niche, stimulating proliferation and neurogenesis, and promotes proliferation and differentiation of retinal progenitor cells into retinal neurons, while in MSCs and pre-osteoblasts it may increase proliferation but decrease osteogenic markers, making betacellulin a valuable reagent for pancreatic, neural and retinal stem cell research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 78%, rat - 75%, bovine - 90%, porcine - 86%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
A GMP-grade version of this protein is available for cell and gene therapy manufacturing. Contact us at customerservice@bioxcell.com for details.
Species reactivity: human. Species similarity: mouse - 78%, rat - 75%, bovine - 90%, porcine - 86%.
Specifications
| Reported Applications |
Cellular proliferation, migration and survival Stimulation of angiogenesis and vascular network development Neurogenesis and neural differentiation Differentiation of retinal progenitor cells |
|---|---|
| Formulation | Lyophilized, white powder |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 9.89 kDa |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in 10 mM HCl (Reconstitution Buffer A) at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |