Catalog #RP019
Recombinant human activin E
Product Description
Recombinant human activin E PLUS™ protein is a dimeric member of the TGF-beta superfamily, formed from two disulfide-linked subunits with the characteristic cystine-knot motif, that regulates cell growth, differentiation and inflammation. Primarily expressed in the liver and also detected in adipose tissue and the gastrointestinal tract, activin E signals through the ALK7 receptor together with ActRIIA and ActRIIB while resisting antagonists such as follistatin, activating the SMAD2/3 pathway in adipocytes. Through its role in glucose and lipid metabolism, adipose regulation, insulin sensitivity and thermogenesis, activin E is a promising target for obesity, diabetes and metabolic disease, and blocking activin E is being explored as an anti-diabetic strategy. Activin E also shares activin functions such as enhancing FSH binding in the ovarian follicle, promoting spermatogenesis and supporting androgen synthesis, making recombinant activin E a versatile reagent for metabolism, endocrinology and inflammation research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: human. Species similarity: mouse - 98%, rat - 98%, bovine - 95%, porcine - 94%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: human. Species similarity: mouse - 98%, rat - 98%, bovine - 95%, porcine - 94%.
Specifications
| Recommended Dilution Buffer | InVivoPure Reconstitution Buffer A |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 23.2 kDa (dimer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in 10 mM HCl (Reconstitution solution A) at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |