Catalog #RP097
Recombinant eel FGF-2 (145 aa) protein
Reactivities
Eel
Applications
Expansion of eel pluripotent, embryonic and mesenchymal stem cells
Serum-free media development
Cellular agriculture and cultivated meat cell culture media optimization
Cellular proliferation, migration and survival
Serum-free media development
Cellular agriculture and cultivated meat cell culture media optimization
Cellular proliferation, migration and survival
Product Description
Recombinant eel FGF-2 (145 aa) protein, also known as basic fibroblast growth factor (bFGF), is a key member of the fibroblast growth factor family, a 145-amino-acid protein of around 16.4 kDa that exists in multiple isoforms and is characterized by a β-trefoil fold stabilized by disulfide bonds essential for biological activity. FGF-2 plays pivotal roles in cell growth, differentiation and angiogenesis, and is a significant growth factor in embryonic development, tissue repair and wound healing. FGF-2 is commonly used in cell culture media to maintain the pluripotency and self-renewal of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), where it activates signaling pathways including the MAPK pathway to sustain expression of the pluripotency-associated genes Oct4, Sox2 and Nanog, preventing differentiation and enabling expansion in vitro. Incorporated into scaffolds or hydrogels alongside stem cells, FGF-2 promotes proliferation and differentiation within a three-dimensional environment, an approach that holds great promise for developing cultivated eel protocols, making eel FGF-2 a key reagent for serum-free media development in cellular agriculture.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: eel.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: eel.
Specifications
| Reported Applications |
Expansion of eel pluripotent, embryonic and mesenchymal stem cells Serum-free media development Cellular agriculture and cultivated meat cell culture media optimization Cellular proliferation, migration and survival |
|---|---|
| Formulation | Lyophilized, white powder |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 16.4 kDa (monomer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |