Catalog #RP052
Recombinant chicken/duck FGF-2 (145 aa) protein
Reactivities
Chicken, Duck
Applications
Chicken and duck primary and stem cell culture
Cellular agriculture process development
Cellular agriculture and cultivated meat cell culture media optimization
Cellular agriculture process development
Cellular agriculture and cultivated meat cell culture media optimization
Product Description
Recombinant chicken/duck FGF-2 (145 aa) protein, also known as basic fibroblast growth factor (bFGF), is a member of the fibroblast growth factor (FGF) family that regulates cell growth, survival, proliferation, migration and differentiation, with critical roles in embryonic development and in adult tissue maintenance, wound healing and angiogenesis. FGF-2 is one of the FGFs that binds and signals through all four tyrosine kinase FGF receptors (FGFR1–4), which recruit SH2- and PTB-domain signaling proteins to activate the RAS/MAP kinase, PI3K/Akt and PLCγ pathways. It is an essential growth factor for maintaining pluripotency in human embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs) and mesenchymal stem cells (MSCs), preserving stemness through PI3K/Akt, ERK1/2, JAK/STAT and PLCγ signaling and activin A activation, and in feeder culture systems it stimulates the production of essential growth factors and cytokines from the feeder layer, making recombinant FGF-2 a key reagent for stem cell culture and pluripotency research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: chicken. Species similarity: duck - 100%.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: chicken. Species similarity: duck - 100%.
Specifications
| Reported Applications |
Chicken and duck primary and stem cell culture Cellular agriculture process development Cellular agriculture and cultivated meat cell culture media optimization |
|---|---|
| Formulation | Lyophilized, white powder |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 16 kDa (monomer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |