Catalog #RP083
Recombinant bovine/porcine FGF2-G3 (154 aa) protein
Reactivities
Porcine, Bovine
Applications
Bovine and porcine stem cell expansion and maintenance
Cellular agriculture and cultivated meat cell culture media optimization
Cellular agriculture and cultivated meat cell culture media optimization
Product Description
Recombinant bovine/porcine FGF2-G3 (154 aa) protein, a form of fibroblast growth factor 2 also known as basic FGF (bFGF), is an essential growth factor for maintaining the pluripotency of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) in feeder-free, chemically defined media and is a core component of widely adopted formulations including mTeSR, StemPro and E8. Native FGF-2 is inherently unstable and prone to proteolytic degradation and aggregation, and its short half-life in culture media (<10 h) contributes to the need for frequent media changes and to challenges in maintaining homogeneity during stem cell proliferation and differentiation. Bovine/porcine FGF2-G3 can serve as a core ingredient of Beefy-9 medium, in which the addition of recombinant albumin to B8 medium enabled the growth of bovine satellite cells and created the first medium to meet the fundamental criteria for a foundational cultured meat medium, notably the complete absence of animal-derived components, making it a key reagent for animal-component-free cell culture and cultivated meat research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: bovine, porcine.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: bovine, porcine.
Specifications
| Reported Applications |
Bovine and porcine stem cell expansion and maintenance Cellular agriculture and cultivated meat cell culture media optimization |
|---|---|
| Formulation | Lyophilized, white powder |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 17.2 kDa (monomer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |