Catalog #RP082
Recombinant bovine/porcine FGF2-G3 (145 aa) protein
Reactivities
Porcine, Bovine
Applications
Maintenance and expansion of bovine iPSC, ESC and primary cells
Cellular agriculture and cultivated meat cell culture media optimization
Cellular agriculture and cultivated meat cell culture media optimization
Product Description
Recombinant bovine/porcine FGF2-G3 (145 aa) protein is a form of FGF-2, also known as basic fibroblast growth factor (bFGF), an essential growth factor for maintaining embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) pluripotency in feeder-free and chemically defined media and a core component of widely adopted media such as mTeSR, StemPro and E8. Native FGF-2 is inherently unstable and prone to proteolytic degradation and aggregation, and its short half-life in culture media (<10 h) contributes to the need for frequent media changes and to challenges in achieving homogeneity during stem cell proliferation and differentiation. Bovine/porcine FGF2-G3 can serve as a core ingredient of Beefy-9, a medium established by Stout et al. in 2023 by adding recombinant albumin to B8 medium for the growth of bovine satellite cells, and the first medium to meet the fundamental criteria of a foundational medium for cultured meat production. Free of animal-derived components, Beefy-9 departs from traditional serum-containing media in support of ethical and sustainable cell culture, making bovine/porcine FGF2-G3 a key reagent for cultivated meat and cellular agriculture research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: bovine, porcine.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: bovine, porcine.
Specifications
| Reported Applications |
Maintenance and expansion of bovine iPSC, ESC and primary cells Cellular agriculture and cultivated meat cell culture media optimization |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 16.3 kDa (monomer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |