Catalog #RP069
Recombinant bovine/porcine FGF-2 (154 aa) protein
Reactivities
Porcine, Bovine
Applications
Bovine and porcine primary cell culture
Bovine and porcine stem cell expansion and maintenance
Cellular agriculture process development
Serum-free media development
Bovine and porcine stem cell expansion and maintenance
Cellular agriculture process development
Serum-free media development
Product Description
Recombinant bovine/porcine FGF-2 (154 aa) protein, also known as basic fibroblast growth factor (bFGF), is a member of the fibroblast growth factor family with a broad range of physiological roles, including regulation of cell proliferation and differentiation. In cell culture, FGF-2 is used to support the maintenance of porcine (pig) and bovine (cow) embryonic stem cells and the proliferation and differentiation of induced pluripotent and mesenchymal stem cells, and it is an essential growth factor in cultivated meat culture media for the proliferation of stem cells and primary cells. Available in two forms, 154 aa and 145 aa, recombinant bovine/porcine FGF-2 facilitates the development of fully optimized serum-free culture media for cellular agriculture applications, making it a key reagent for cultivated meat and cellular agriculture research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: bovine, porcine.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: bovine, porcine.
Specifications
| Reported Applications |
Bovine and porcine primary cell culture Bovine and porcine stem cell expansion and maintenance Cellular agriculture process development Serum-free media development |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 17.1 kDa |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |