Catalog #RP057
Recombinant bovine/porcine FGF-2 (145 aa) protein
Reactivities
Porcine, Bovine
Applications
Bovine and porcine primary cell culture
Bovine and porcine stem cell expansion and maintenance
Cellular agriculture process development
Bovine and porcine stem cell expansion and maintenance
Cellular agriculture process development
Product Description
Recombinant bovine/porcine FGF-2 (145 aa) protein, also known as basic fibroblast growth factor (bFGF), is a member of the fibroblast growth factor (FGF) family that regulates cell growth, survival, proliferation, migration and differentiation, with critical roles in embryonic development and in adult tissue maintenance, wound healing and angiogenesis. FGF-2 is one of the FGFs that binds and signals through all four tyrosine kinase FGF receptors (FGFR1–4), which recruit SH2- and PTB-domain signaling proteins to activate the RAS/MAP kinase, PI3K/Akt and PLCγ pathways. It is an essential growth factor for maintaining pluripotency in human embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs) and mesenchymal stem cells (MSCs), preserving stemness through PI3K/Akt, ERK1/2, JAK/STAT and PLCγ signaling and activin A activation, and in feeder culture systems it stimulates the production of essential growth factors and cytokines from the feeder layer, making recombinant FGF-2 a key reagent for stem cell culture and pluripotency research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: bovine, porcine.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: bovine, porcine.
Specifications
| Reported Applications |
Bovine and porcine primary cell culture Bovine and porcine stem cell expansion and maintenance Cellular agriculture process development |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 16 kDa |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |