Catalog #RP081
Recombinant bovine PDGF-BB protein
Reactivities
Bovine
Applications
Culture of bovine (cow) muscle stem cells
Cellular agriculture and cultivated meat cell culture media optimization
Serum-free media development
Proliferation of fibroblasts and smooth muscle cells
Cellular agriculture and cultivated meat cell culture media optimization
Serum-free media development
Proliferation of fibroblasts and smooth muscle cells
Product Description
Recombinant bovine PDGF-BB protein (platelet-derived growth factor BB) is a 24.5 kDa homodimer of two disulfide-linked PDGF-B chains and a member of the PDGF family of A, B, C and D chains, with a conserved cystine knot motif crucial for its stability and function. PDGF-BB binds the tyrosine kinase receptors PDGFR-α and PDGFR-β, inducing receptor dimerization and autophosphorylation and activating the PI3K/Akt, MAPK and PLCγ pathways that regulate proliferation, migration, survival and differentiation. In wound healing and development, PDGF-BB stimulates proliferation and migration of fibroblasts and smooth muscle cells, promotes granulation tissue formation and extracellular matrix remodeling, and enhances angiogenesis by recruiting pericytes and smooth muscle cells to stabilize new blood vessels. In cultivated meat manufacturing, PDGF-BB promotes rapid cell expansion in bioreactors, aids differentiation of myoblasts into mature muscle cells, improves cell migration and integration within scaffolds, and stimulates extracellular matrix production, while reducing reliance on fetal bovine serum (FBS), making it a key growth factor for cost-effective, serum-free cultivated meat production.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: bovine.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: bovine.
Specifications
| Reported Applications |
Culture of bovine (cow) muscle stem cells Cellular agriculture and cultivated meat cell culture media optimization Serum-free media development Proliferation of fibroblasts and smooth muscle cells |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 24.5 kDa (monomer) |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in sterile-filtered water at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |