Catalog #RP070
Recombinant bovine HGF NK1 protein
Reactivities
Bovine
Applications
Cellular agriculture process development
Differentiation of iPSCs to hepatocyte-like cells
Cellular agriculture and cultivated meat cell culture media optimization
iPSC and primary cell differentiation to muscle
Differentiation of iPSCs to hepatocyte-like cells
Cellular agriculture and cultivated meat cell culture media optimization
iPSC and primary cell differentiation to muscle
Product Description
Recombinant bovine HGF NK1 protein (hepatocyte growth factor NK1 isoform) is the smallest naturally occurring splice variant of HGF, comprising the N-terminus and first kringle domain of a growth factor that regulates cell growth, motility and morphogenesis and plays a central role in angiogenesis, tumorigenesis and tissue regeneration. HGF binds and activates the receptor tyrosine kinase c-Met to trigger PI3K/AKT, FAK, JNK and ERK1/2 signaling, and bovine HGF shows higher solubility, stability and receptor binding affinity than human HGF. In muscle, bovine HGF drives satellite cell proliferation, enhances myoblast formation and fusion, increases myofibrillar protein synthesis and helps restore damaged muscle structure, with further activity in liver, brain and kidney. In cell culture, HGF improves proliferation, differentiation, attachment and survival of skeletal muscle cells and cardiomyocytes and supports serum-free media optimization, and this HGF NK1 is produced in an animal-free microbial system compatible with production at scale, making it a key growth factor for cultivated meat and cellular agriculture research.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: bovine.
This protein is optimized for in vivo and functional applications: low-endotoxin, tag-free, and carrier-free, supporting reproducible performance in demanding preclinical and bioactivity studies.
Species reactivity: bovine.
Specifications
| Reported Applications |
Cellular agriculture process development Differentiation of iPSCs to hepatocyte-like cells Cellular agriculture and cultivated meat cell culture media optimization iPSC and primary cell differentiation to muscle |
|---|---|
| Formulation | Lyophilized, white powder |
| Endotoxin |
≤0.5 EU/mg LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | Sterile |
| Production | Microbial. Expressed in E. coli |
| Molecular Weight | 20 kDa |
| Storage | Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles |
| Mycoplasma | Negative |
| Bioactivity Assay | No significant difference between EC50 of reference and test lots |
| Reconstitution | Resuspend in 10 mM HCl (Reconstitution Buffer A) at >50 µg/ml. Once reconstituted, store in single-use aliquots to avoid freeze-thaw cycles. Store reconstituted protein at -20 °C to -80 °C for a maximum of 12 months or until the expiry date if sooner. |
| Shelf Life | This product has a guaranteed shelf-life of one year from the date of customer receipt when stored as recommended. |