Catalog #CP075
RecombiMAb anti-mouse NKG2AB6 (LALA-PG)
Clone
16A11-CP075
Reactivities
Mouse
Isotype
Mouse IgG2a LALA-PG
You may also be interested in:
Product Description
16A11-CP075 monoclonal antibody is a recombinant, chimeric version of the original 16A11 antibody. The variable domain sequences are identical but the constant region has been switched from mouse IgG2b to mouse IgG2a. 16A11-CP075 also contains the Fc silencing mutation LALA-PG rendering it unable to bind to endogenous Fcγ receptors thus preventing effector functions such as antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). The highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies. The 16A11 antibody reacts with NKG2A from C57BL/6 mice and does not react with BALB/c or 129 mouse strains. NKG2A, also known as CD159a is a type II transmembrane glycoprotein which belongs to the killer cell lectin-like receptor (KLR) family. NKG2A is expressed on NK cells, NKT cells, and activated CD8 T cells. NKG2A forms a disulfide-bonded heterodimer with CD94 that can bind to non-classical MHC class I antigen Qa-1 on target cells and inhibit NK cell activation.
Specifications
| Isotype | Mouse IgG2a LALA-PG |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
| Mutations | LALA-PG |
| Immunogen | C57BL/6 mouse CD94/NKG2A transfected CHO cells |
| Reported Applications | Flow Cytometry |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL gel clotting assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from mammalian cell supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |