Catalog #CP203
RecombiMAb anti-mouse/human/rat/monkey ICOS (CD278)
Clone
C398.4A-CP203
Reactivities
Mouse, Human, Rat, Monkey
Applications
ELISA
in vitro T cell stimulation/activation*
Flow cytometry*
Immunoprecipitation*
*Reported for the original C398.4A antibody
in vitro T cell stimulation/activation*
Flow cytometry*
Immunoprecipitation*
*Reported for the original C398.4A antibody
Isotype
Mouse IgG2a, κ (switched from Armenian hamster IgG)
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Product Description
The C398.4A-CP203 monoclonal antibody is a chimeric version of the original C398.4A antibody. The variable domain sequences are identical but the constant region sequences have been switched from Armenian hamster IgG to Mouse IgG2a, κ. The C398.4A-CP203 antibody has an effector function competent Fc domain allowing for activation of Fcγ receptors (FcγRs) to trigger antibody‑dependent cellular cytotoxicity (ADCC), antibody‑dependent cellular phagocytosis (ADCP), complement‑dependent cytotoxicity (CDC) and opsonization to promote target cell depletion. The mouse IgG2a isotype demonstrates strong effector functions due to potent interaction with mFcγRIV, which is functionally similar to the FcγRIIIa receptor involved in human ADCC. Species-matched chimeric antibodies result in less immunogenicity and formation of anti-drug antibodies (ADAs) than xenogenic antibodies in animal models. The highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies.
The C398.4A monoclonal antibody reacts with mouse, human, rat and monkey ICOS (inducible T cell co-stimulator), also known as CD278. ICOS is a 47-57 kDa homodimeric glycoprotein belonging to the CD28 family of costimulatory molecules. ICOS is expressed on activated T cells and upon ICOSL binding, co-stimulates T and B cell responses. The ligand Is expressed on antigen presenting cells including splenic B cells, dendritic cells, and macrophages. ICOS signaling is also thought to be important for maintaining regulatory T cell homeostasis. The C398.4A antibody is commonly used to co-stimulate T cell activation and proliferation in vitro. C398.4A does not block the binding of ICOS to ICOSL.
The C398.4A monoclonal antibody reacts with mouse, human, rat and monkey ICOS (inducible T cell co-stimulator), also known as CD278. ICOS is a 47-57 kDa homodimeric glycoprotein belonging to the CD28 family of costimulatory molecules. ICOS is expressed on activated T cells and upon ICOSL binding, co-stimulates T and B cell responses. The ligand Is expressed on antigen presenting cells including splenic B cells, dendritic cells, and macrophages. ICOS signaling is also thought to be important for maintaining regulatory T cell homeostasis. The C398.4A antibody is commonly used to co-stimulate T cell activation and proliferation in vitro. C398.4A does not block the binding of ICOS to ICOSL.
Specifications
| Isotype | Mouse IgG2a, κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb mouse IgG2a isotype control, unknown specificity |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | Mouse T cell line D10.G4.1 |
| Reported Applications |
ELISA in vitro T cell stimulation/activation* Flow cytometry* Immunoprecipitation* *Reported for the original C398.4A antibody |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from mammalian cell supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |