RecombiMAb anti-mouse FAP
Catalog #CP057
Clone:
73.3-CP057
Isotype:
Mouse IgG2a,Ā Īŗ
(switched from mouse Mouse IgG1,Ā Īŗ)
(switched from mouse Mouse IgG1,Ā Īŗ)
Product Details
The CP057 monoclonal antibody is a recombinant, chimeric version of the original 73.3 antibody. The variable domain sequences are identical but the constant region sequences have been switched from Mouse IgG1, Īŗ to Mouse IgG2a, Īŗ. The highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies. The 73.3 monoclonal antibody reacts with mouse fibroblast activation protein (FAP), a cell-surface serine protease that acts on various hormones and extracellular matrix components. FAP is expressed during embryonic development, in tissues of healing wounds, and in chronic inflammatory and fibrotic conditions. FAP protein is highly upregulated in cancer-associated fibroblasts (CAFs) in epithelial tumors. CAF overexpression of FAP promotes tumor development and metastasis by influencing extracellular matrix remodeling, intracellular signaling, angiogenesis, epithelial-to-mesenchymal transition, and immunosuppression. The scFv of the 73.3 antibody has been used to construct 73.3-FAP-CAR T cells specific for FAP. Adoptively transferred 73.3-FAP-CAR T cells have been shown to inhibit the growth of multiple syngeneic mouse tumor models.Specifications
| Isotype | Mouse IgG2a,Ā Īŗ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb mouse IgG2a isotype control, unknown specificity |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
| Immunogen | Immunization of FAP-null mice with FAP-expressing 3T3 cells |
| Reported Applications |
Immunoprecipitation Flow cytometry Western blot Chimeric Antigen Receptor construction *Reported for the original 73.3 antibody. For information on in vivo applications, please contact technicalservice@bioxcell.com |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<1EU/mg (<0.001EU/Ī¼g) Determined by LAL gel clotting assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 Āµm filtration |
| Production | Purified from HEK293 cell supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theilerās Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
Additional Formats
Recommended Products
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Recommended Isotype Control(s)
RecombiMAb mouse IgG2a isotype control, unknown specificity
-
Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
Please see references for the original mouse IgG1 73.3 antibody (https://bioxcell.com/invivomab-anti-mouse-fap-be0374).