RecombiMAb anti-mouse EGFR (domain-II)
Catalog #CP068
Clone:
EMab-301-CP068
Reactivities:
Mouse
Isotype:
Rat IgG2a, Īŗ
(converted from Rat IgM, Īŗ)
(converted from Rat IgM, Īŗ)
Product Details
The EMab-301-CP068 monoclonal antibody is a recombinant version of the original EMab-301 antibody. The variable domain sequences are identical but the constant region sequences have been converted from rat IgM to rat IgG2a. EMab-301-CP068 selectively binds extracellular domain II of mouse EGFR (epidermal growth factor receptor). Domain II is a critical segment of the receptor's extracellular region responsible for mediating receptor dimerization and activation upon ligand binding. Mouse EGFR (also known as ERBB, ERBB1, and HER1) is a transmembrane glycoprotein and a member of the ErbB family of receptor protein tyrosine kinases (RTKs). EGFR ligands include EGF, AREG, TGF-Ī±, epiregulin (EREG), epigen, betacellulin, and heparin-binding EGF. Upon ligand binding, EGFR dimerizes and is activated through autophosphorylation of the cytoplasmic domain. EGFR activation triggers several downstream signaling pathways, including RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLC-Ī³-PKC, and STATs which help the cell respond accurately to extracellular signals. EGFR is expressed ubiquitously in various tissues and plays critical roles in developmental, physiological, and regenerative processes. The aberrant activation of EGFR is a common feature of various malignancies.Specifications
| Isotype | Rat IgG2a,Ā Īŗ |
|---|---|
| Recommended Isotype Control(s) | InVivoPlus rat IgG2a isotype control, anti-trinitrophenol |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
| Immunogen | mouse EGFR overexpressing cells |
| Reported Applications |
Western Blot For information on applications, please contact (technicalservice@bioxcell.com) |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<1EU/mg (<0.001EU/Ī¼g) Determined by LAL gel clotting assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 Āµm filtration |
| Production | Purified from mammalian cell supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theilerās Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
Recommended Products
-
Recommended Isotype Control(s)
InVivoPlus rat IgG2a isotype control, anti-trinitrophenol
-
Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer