Catalog #CP212
RecombiMab anti-mouse CD71 (TfR1) (LALA-PG)
Clone
R17 217.1.3-CP212
Reactivities
Mouse
Applications
ELISA
Antibody-oligonucleotide conjugate (AOC) synthesis
Antibody-oligonucleotide conjugate (AOC) synthesis
Isotype
Mouse IgG2a (LALA-PG), κ
(Switched from Rat IgG2a, κ)
(Switched from Rat IgG2a, κ)
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Product Description
The R17 217.1.3-CP212 monoclonal antibody is a recombinant, chimeric version of the original R17 217.1.3 antibody. The variable domain sequences are identical but the constant region sequences have been switched from Rat IgG2a, κ to mouse IgG2a, κ for use in murine models. Additionally, R17 217.1.3-CP212 includes the Fc silencing mutation variant LALA-PG, rendering it unable to bind endogenous murine Fcγ receptors or C1q to induce antibody-dependent cell-mediated cytotoxicity (ADCC) or complement-dependent cytotoxicity (CDC). Studies have demonstrated administration of anti-TfR1 antibodies with full Fc effector function in mice resulted in acute clinical signs of toxicity and a decrease in reticulocyte count. These adverse effects were eliminated through silencing of the Fc region of the antibody. The LALA-PG variant has demonstrated significantly reduced effector function, C1q binding and C3 fixation while retaining favorable biophysical and manufacturing properties and preserving FcRn-mediated half-life. Species-matched chimeric antibodies demonstrate reduced immunogenicity and formation of anti-drug antibodies (ADAs) compared to xenogenic antibodies in animal models. The highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies.
The R17 217.1.3/TIB-219 monoclonal antibody reacts with mouse CD71 also known as transferrin receptor protein 1 (TfR1). CD71 is a 170-180 kDa type II homodimeric transmembrane glycoprotein which is expressed on the surface of proliferating cells, reticulocytes, and erythroid precursors. CD71 plays a role in the control of cellular proliferation and is required for iron import from transferrin into cells by endocytosis. Due to its important role in proliferation and cellular iron intake as well as the fact that many cancerous cells express high levels of CD71 it is being explored as a potential new target in cases of human leukemia & lymphoma. The R17 217.1.3 antibody is frequently used for synthesis of antibody-oligonucleotide conjugates (AOCs) in research involving targeted delivery of pre-clinical therapeutic oligonucleotides or to improve detection sensitivity in experimental molecular diagnostics.
The R17 217.1.3/TIB-219 monoclonal antibody reacts with mouse CD71 also known as transferrin receptor protein 1 (TfR1). CD71 is a 170-180 kDa type II homodimeric transmembrane glycoprotein which is expressed on the surface of proliferating cells, reticulocytes, and erythroid precursors. CD71 plays a role in the control of cellular proliferation and is required for iron import from transferrin into cells by endocytosis. Due to its important role in proliferation and cellular iron intake as well as the fact that many cancerous cells express high levels of CD71 it is being explored as a potential new target in cases of human leukemia & lymphoma. The R17 217.1.3 antibody is frequently used for synthesis of antibody-oligonucleotide conjugates (AOCs) in research involving targeted delivery of pre-clinical therapeutic oligonucleotides or to improve detection sensitivity in experimental molecular diagnostics.
Specifications
| Isotype | Mouse IgG2a (LALA-PG), κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | LALA-PG |
| Immunogen | Mouse erythroleukemia cell line 745.6 |
| Reported Applications |
ELISA Antibody-oligonucleotide conjugate (AOC) synthesis |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from HEK293 cell supernatant in an animal-free facility |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |