Catalog #CP111
RecombiMAb anti-mouse CD39 (Entpd1) (LALA-PG)
Clone
C39Mab-1-CP111
Reactivities
Mouse
Isotype
Mouse IgG2a (LALA-PG), κ
(switched from Rat Ig2a, κ)
(switched from Rat Ig2a, κ)
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Product Description
The C39Mab-1-CP111 monoclonal antibody is a recombinant, chimeric version of the original C39Mab-1 antibody. The variable domain sequences are identical but the constant region sequences have been switched from Rat IgG2a, κ to mouse IgG2a, κ for use in murine models. Additionally, C39Mab-1-CP111 contains LALA-PG mutations in the heavy chain Fc fragment rendering it unable to bind endogenous murine Fcγ receptors or C1q to induce antibody-dependent, cell-mediated cytotoxicity (ADCC) or complement-dependent cytotoxicity (CDC). The LALA-PG variant has demonstrated significantly reduced effector function, C1q binding and C3 fixation compared to other common silencing mutations such as the LALA and DANG variants while retaining favorable biophysical and manufacturing properties. Species-matched chimeric antibodies demonstrate reduced immunogenicity and formation of anti-drug antibodies (ADAs) compared to xenogenic antibodies in animal models. The highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies.
The C39Mab-1 monoclonal antibody reacts with mouse CD39, also known as ectonucleoside triphosphate diphosphohydrolase 1 (Entpd1), NTPDase-1, ATP-DPH, and Ecto-ATPase 1. The major function of CD39 is to hydrolyze extracellular adenosine triphosphate (eATP) to diphosphate (eADP) and adenosine monophosphate (AMP). 5’-nucleotidase (NT5E/CD73) dephosphorylates AMP to generate adenosine, which mediates an immunosuppressive tumor microenvironment in tumors. CD39 acts as an "immunological switch" by regulating the balance between immunostimulatory or pro-inflammatory eATP and immunosuppressive adenosine nucleotides, thereby controlling inflammation and immune responses. This function is critical in various processes, including immune cell regulation, anti-tumor immunity, and vascular inflammation.
The C39Mab-1 monoclonal antibody reacts with mouse CD39, also known as ectonucleoside triphosphate diphosphohydrolase 1 (Entpd1), NTPDase-1, ATP-DPH, and Ecto-ATPase 1. The major function of CD39 is to hydrolyze extracellular adenosine triphosphate (eATP) to diphosphate (eADP) and adenosine monophosphate (AMP). 5’-nucleotidase (NT5E/CD73) dephosphorylates AMP to generate adenosine, which mediates an immunosuppressive tumor microenvironment in tumors. CD39 acts as an "immunological switch" by regulating the balance between immunostimulatory or pro-inflammatory eATP and immunosuppressive adenosine nucleotides, thereby controlling inflammation and immune responses. This function is critical in various processes, including immune cell regulation, anti-tumor immunity, and vascular inflammation.
Specifications
| Isotype | Mouse IgG2a (LALA-PG), κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | LALA-PG |
| Immunogen | LN229 cells over-expressing mouse CD39 |
| Reported Applications |
Flow cytometry Western blot For details on in vivo applications please contact technicalservice@bioxcell.com |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from mammalian cell supernatant in an animal-free facility |
| Purification | Protein A |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |