Catalog #CP227
RecombiMAb anti-mouse CD2 (LALA-PG)
Clone
RM2-5-CP227
Reactivities
Mouse
Applications
in vivo CD2 blockade*
in vitro CD2 blockade*
in vivo antibody-mediated cell targeting for therapeutic nanoparticle delivery*
Functional Assay*
Flow cytometry*
Immunoprecipitation*
*Reported for the original Rat IgG2b RM2-5 antibody
in vitro CD2 blockade*
in vivo antibody-mediated cell targeting for therapeutic nanoparticle delivery*
Functional Assay*
Flow cytometry*
Immunoprecipitation*
*Reported for the original Rat IgG2b RM2-5 antibody
Isotype
Mouse IgG2a (LALA-PG), λ
(Switched from Rat IgGb, λ)
(Switched from Rat IgGb, λ)
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Product Description
The RM2-5-CP227 antibody is a chimeric version of the original RM2-5 antibody. The variable domain sequences are identical but the constant region sequences have been switched from rat IgG2b to mouse IgG2a. Additionally, the constant region includes the Fc silencing mutation variant LALA-PG rendering it unable to bind endogenous murine Fcγ receptors or C1q to induce antibody-dependent cell-mediated cytotoxicity (ADCC) or complement-dependent cytotoxicity (CDC). This is useful to study the effects of CD2 blockade and targeting—without FcγR/complement‑mediated cell killing, immune activation, or Fc‑driven biodistribution artifacts enabling clean mechanistic dissection. Fc-silent antibodies can also improve in-vitro assays by reducing non-specific signaling and background. The LALA-PG variant has demonstrated significantly reduced effector function, C1q binding and C3 fixation compared to other common silencing mutations while retaining favorable biophysical and manufacturing properties and preserving FcRn-mediated half-life. Species-matched chimeric antibodies result in less immunogenicity and formation of anti-drug antibodies (ADAs) than xenogenic antibodies in animal models and the highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies.
The RM2-5 monoclonal antibody reacts with mouse CD2, a 50–55 kDa type I transmembrane glycoprotein and member of the immunoglobulin superfamily. CD2 is also called lymphocyte function-associated antigen 2 (LFA-2), ant T-11. CD2 is expressed on thymocytes, mature CD4⁺ and CD8⁺ T cells, NK cells, NKT cells, and on murine B cells. The cognate ligand of CD2 in mouse is CD48 (the human ortholog binds CD58/LFA-3). CD2/CD48 engagement mediates cell–cell adhesion at the immunological synapse, lowers the threshold for T-cell receptor (TCR)–mediated activation, supports NK-cell cytotoxicity, and contributes to memory T-cell maintenance. CD2-deficient mice exhibit altered thymocyte selection and impaired T-cell activation, and CD2-CD48 interactions are involved in the pathophysiology of allograft rejection, graft-versus-host disease, autoimmune diseases (e.g., psoriasis, rheumatoid arthritis, multiple sclerosis), T-cell malignancies, and chronic viral infections. Antibody-based blockade of CD2-mediated adhesion and co-stimulation is emerging as a therapeutic strategy in transplantation, autoimmunity, and oncology, with clinical-stage anti-human CD2 agents (e.g., alefacept, siplizumab) validating the target. The RM2-5 antibody is suitable for flow cytometric characterization, immunoprecipitation, and in vivo modulation of CD2 in murine models of immune activation, autoimmunity, transplantation, and tumor immunology.
NOTE: Please see Yagita et al. Proc Natl Acad Sci USA 1989;86(2):645–9 (PMID 2463634) for the foundational characterization of the RM2-series anti-mouse CD2 antibodies, including identification of CD2 expression on B cells.
The RM2-5 monoclonal antibody reacts with mouse CD2, a 50–55 kDa type I transmembrane glycoprotein and member of the immunoglobulin superfamily. CD2 is also called lymphocyte function-associated antigen 2 (LFA-2), ant T-11. CD2 is expressed on thymocytes, mature CD4⁺ and CD8⁺ T cells, NK cells, NKT cells, and on murine B cells. The cognate ligand of CD2 in mouse is CD48 (the human ortholog binds CD58/LFA-3). CD2/CD48 engagement mediates cell–cell adhesion at the immunological synapse, lowers the threshold for T-cell receptor (TCR)–mediated activation, supports NK-cell cytotoxicity, and contributes to memory T-cell maintenance. CD2-deficient mice exhibit altered thymocyte selection and impaired T-cell activation, and CD2-CD48 interactions are involved in the pathophysiology of allograft rejection, graft-versus-host disease, autoimmune diseases (e.g., psoriasis, rheumatoid arthritis, multiple sclerosis), T-cell malignancies, and chronic viral infections. Antibody-based blockade of CD2-mediated adhesion and co-stimulation is emerging as a therapeutic strategy in transplantation, autoimmunity, and oncology, with clinical-stage anti-human CD2 agents (e.g., alefacept, siplizumab) validating the target. The RM2-5 antibody is suitable for flow cytometric characterization, immunoprecipitation, and in vivo modulation of CD2 in murine models of immune activation, autoimmunity, transplantation, and tumor immunology.
NOTE: Please see Yagita et al. Proc Natl Acad Sci USA 1989;86(2):645–9 (PMID 2463634) for the foundational characterization of the RM2-series anti-mouse CD2 antibodies, including identification of CD2 expression on B cells.
Specifications
| Isotype | Mouse IgG2a, λ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | LALA-PG |
| Immunogen | BALB/c mouse thymocytes |
| Reported Applications |
in vivo CD2 blockade* in vitro CD2 blockade* in vivo antibody-mediated cell targeting for therapeutic nanoparticle delivery* Functional Assay* Flow cytometry* Immunoprecipitation* *Reported for the original Rat IgG2b RM2-5 antibody |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from mammalian cell supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |