Catalog #CP115
RecombiMAb anti-mouse CCR6 (LALA-PG)
Clone
C6Mab-13-CP115
Reactivities
Mouse
Isotype
Mouse IgG2a (LALA-PG), κ
(switched from Rat IgG1, κ)
(switched from Rat IgG1, κ)
Product Description
The C6Mab-13-CP115 monoclonal antibody is a recombinant, chimeric version of the original C6Mab-13 antibody. The variable domain sequences are identical but the constant region sequences have been switched from Rat IgG2b, κ to mouse IgG2a, κ for use in murine models. Additionally, C6Mab-13-CP115 contains LALA-PG mutations in the heavy chain Fc fragment rendering it unable to bind endogenous murine Fcγ receptors or C1q to induce antibody-dependent, cell-mediated cytotoxicity (ADCC) or complement-dependent cytotoxicity (CDC). The LALA-PG variant has demonstrated significantly reduced effector function, C1q binding and C3 fixation compared to other common silencing mutations such as the LALA and DANG variants while retaining favorable biophysical and manufacturing properties. Species-matched chimeric antibodies demonstrate reduced immunogenicity and formation of anti-drug antibodies (ADAs) compared to xenogenic antibodies in animal models. The highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies.
Mouse CCR6 (Ccr6) is a G protein–coupled chemokine receptor expressed on multiple immune cell subsets, including dendritic cells, B cells, and distinct T cell populations such as Th17 cells. It binds the chemokine CCL20 and directs cell trafficking to sites of inflammation, particularly in mucosal tissues like the gut and lung, where it contributes to host defense against microbes. Genetic disruption of CCR6 in mice leads to altered lymphocyte and dendritic cell localization and blunted inflammatory responses, highlighting its central role in shaping tissue immune microenvironments.
Mouse CCR6 (Ccr6) is a G protein–coupled chemokine receptor expressed on multiple immune cell subsets, including dendritic cells, B cells, and distinct T cell populations such as Th17 cells. It binds the chemokine CCL20 and directs cell trafficking to sites of inflammation, particularly in mucosal tissues like the gut and lung, where it contributes to host defense against microbes. Genetic disruption of CCR6 in mice leads to altered lymphocyte and dendritic cell localization and blunted inflammatory responses, highlighting its central role in shaping tissue immune microenvironments.
Specifications
| Isotype | Mouse IgG2a (LALA-PG), κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | LALA-PG |
| Immunogen | mCCR6p1-19C peptide |
| Reported Applications |
Flow cytometry For details on in vivo applications please contact technicalservice@bioxcell.com |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation* |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from mammalian cell supernatant in an animal-free facility |
| Purification | Protein A |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |
* Additional quality control measures for our InVivoPlusâ„¢ products include advanced binding validation, murine pathogen screening, protein aggregation screening, and ultra-low endotoxin levels. The superior quality of our InVivoPlusâ„¢ products will meet and exceed the strict demands and rigorous standards required for in vivo research. Learn more about the InVivoPlusâ„¢ difference here.