Catalog #CP112
RecombiMAb anti-mouse CCR1
Clone
C1Mab-6-CP112
Reactivities
Mouse
Applications
Flow Cytometry*
Western Blot*
*Reported for the original C1Mab-6 antibody
Western Blot*
*Reported for the original C1Mab-6 antibody
Isotype
Mouse IgG2a, κ (switched from Rat IgG2b, κ)
Product Description
The C1Mab-6-CP112 monoclonal antibody is a chimeric version of the original C1Mab-6 antibody. The variable domain sequences are identical but the constant region sequences have been switched from Rat IgG2b, κ to Mouse IgG2a, κ. The C1Mab-6-CP112 antibody has an effector function competent Fc domain allowing for activation of Fcγ receptors (FcγRs) to trigger antibody‑dependent cellular cytotoxicity (ADCC), antibody‑dependent cellular phagocytosis (ADCP), complement‑dependent cytotoxicity (CDC) and opsonization to promote target cell depletion. The mouse IgG2a isotype demonstrates strong effector functions due to potent interaction with mFcγRIV, which is functionally similar to the FcγRIIIa receptor involved in human ADCC. Species-matched chimeric antibodies result in less immunogenicity and formation of anti-drug antibodies (ADAs) than xenogenic antibodies in animal models. The highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies.
C1Mab‑6-CP112 specifically targets mouse CCR1 (C‑C chemokine receptor type 1), a G‑protein‑coupled chemokine receptor that mediates cell migration and inflammatory responses by binding specific CC chemokines such as CCL3 (MIP‑1α) and CCL5 (RANTES). Mouse CCR1 is expressed on monocytes, macrophages, dendritic cells, neutrophils, mast cells, and subsets of T cells and B cells, depending on activation and tissue context. Mouse CCR1 is implicated in a broad range of inflammatory, fibrotic, and some neoplastic disease models, mainly through its role in leukocyte recruitment and macrophage/monocyte activation. Mouse CCR1 is ~80% homologous to human CCR1, making mouse models useful for studying human‑like inflammatory and immune‑pathology pathways.
C1Mab‑6-CP112 specifically targets mouse CCR1 (C‑C chemokine receptor type 1), a G‑protein‑coupled chemokine receptor that mediates cell migration and inflammatory responses by binding specific CC chemokines such as CCL3 (MIP‑1α) and CCL5 (RANTES). Mouse CCR1 is expressed on monocytes, macrophages, dendritic cells, neutrophils, mast cells, and subsets of T cells and B cells, depending on activation and tissue context. Mouse CCR1 is implicated in a broad range of inflammatory, fibrotic, and some neoplastic disease models, mainly through its role in leukocyte recruitment and macrophage/monocyte activation. Mouse CCR1 is ~80% homologous to human CCR1, making mouse models useful for studying human‑like inflammatory and immune‑pathology pathways.
Specifications
| Isotype | Mouse IgG2a, κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb mouse IgG2a isotype control, unknown specificity |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | CHO cells overexpressing mouse CCR1 (CHO/mCCR1) |
| Reported Applications |
Flow Cytometry* Western Blot* *Reported for the original C1Mab-6 antibody |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from mammalian cell supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |