Catalog #CP065
RecombiMAb anti-human CD71 (TfR1)
Clone
B3/25-CP065
Reactivities
Human
Applications
Western Blot
in vitro CD71 targeting*
in vivo CD71 targeting*
Immunofluorescence*
*Reported for the original Mouse IgG1, κ antibody. For information on in vivo applications, please contact technicalservice@bioxcell.com
in vitro CD71 targeting*
in vivo CD71 targeting*
Immunofluorescence*
*Reported for the original Mouse IgG1, κ antibody. For information on in vivo applications, please contact technicalservice@bioxcell.com
Isotype
Mouse IgG2a, κ
(Switched from Mouse IgG1, κ)
(Switched from Mouse IgG1, κ)
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Product Description
The B3/25-CP065 monoclonal antibody is a chimeric version of the original B3/25 antibody. The variable domain sequences are identical but the constant region sequences have been switched from mouse IgG1 to mouse IgG2a. The mouse IgG2a isotype demonstrates strong effector functions due to potent interaction with mFcγRIV, which is functionally similar to the FcγRIIIa receptor involved in human ADCC. The B3/25 monoclonal antibody reacts with human CD71 also known as transferrin receptor or TfR1. CD71 is a type II homodimeric transmembrane glycoprotein which is expressed on the surface of proliferating cells, reticulocytes, and erythroid precursors. CD71 plays a role in the control of cellular proliferation and is required for iron import from transferrin into cells. CD71 is expressed on malignant cells at high levels and its expression correlates with cancer progression. The B3/25 antibody is a non-neutralizing antibody that does not inhibit the binding of transferrin but does result in decreased transferrin binding sites. B3/25 has been shown to inhibit the in vitro proliferation of human myeloid leukemia cells as well as used for the delivery of toxins into cancer cells. In vivo studies carried out in nude mice showed that one intravenous injection of B3/25 antibody alone inhibits growth of human melanoma M21 xenografts.
Specifications
| Isotype | Mouse IgG2a, κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb mouse IgG2a isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
| Immunogen | Human hematopoietic cell line K562 |
| Reported Applications |
Western Blot in vitro CD71 targeting* in vivo CD71 targeting* Immunofluorescence* *Reported for the original Mouse IgG1, κ antibody. For information on in vivo applications, please contact technicalservice@bioxcell.com |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from mammalian cell supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |