Catalog #CP247
RecombiMAb anti-human CD7
Clone
TH69-CP247
Reactivities
Human
Applications
in vivo CD7 blockade*
in vivo therapy (CD7⁺ tumor cell elimination) *
in vivo radioimmunodetection*
in vitro complement-dependent cytotoxicity*
*Baum et al., Br J Haematol 1996 · PMID 8904888
Flow cytometry*
Immunofluorescence*
Epitope competition (scFvCD7-GFP)*
in vitro targeted cytotoxicity*
*Peipp et al., Cancer Res 2002 · PMID 12019163
in vivo therapy (CD7⁺ tumor cell elimination) *
in vivo radioimmunodetection*
in vitro complement-dependent cytotoxicity*
*Baum et al., Br J Haematol 1996 · PMID 8904888
Flow cytometry*
Immunofluorescence*
Epitope competition (scFvCD7-GFP)*
in vitro targeted cytotoxicity*
*Peipp et al., Cancer Res 2002 · PMID 12019163
Isotype
Mouse IgG1, κ
Product Description
The TH69-CP247 monoclonal antibody is a recombinant mouse IgG1, κ isotype variant of the anti-human CD7 clone TH69 that reacts with human CD7. The parental TH69 hybridoma was generated at the University of Erlangen-Nürnberg by immunization with the human T-cell acute lymphoblastic leukemia lines CEM and HSB-2, and shows little reactivity outside the T lineage. CD7 is a 40 kDa type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. CD7 is also known as GP40, LEU-9, TP41, and Tp40. It is expressed on the majority of thymocytes, mature T cells, and NK cells, as well as on early lymphoid progenitors, where it is one of the earliest surface markers of T-lineage commitment. CD7 has been reported to interact with SECTM1 (K12) and galectin-1 and plays roles in T and NK cell co-stimulation, adhesion, and activation. CD7 is highly expressed in T-cell acute lymphoblastic leukemia (T-ALL), T-cell lymphomas, and a subset of acute myeloid leukemias (AML), while being largely absent from non-hematopoietic tissues. Because of this expression profile, CD7 is considered an attractive target for antibody-, immunotoxin-, and CAR-T cell-based therapies for T-cell malignancies, where CD7 expression on normal T cells necessitates strategies to avoid CAR-T fratricide. A defining feature of the TH69 clone is that antigen binding triggers rapid internalization of the CD7-antibody complex into the endosomal and lysosomal compartment, a property that has been exploited in scFv immunotoxin, antibody-drug conjugate, and targeted payload delivery formats. TH69 recognizes human CD7 and is not expected to cross-react with mouse CD7, which lacks the proline-rich extracellular repeats present in the human protein. Unlike the original hybridoma-derived clone TH69, clone TH69-CP247 features the TH69 variable region on a mouse IgG1, κ backbone, providing sequence-defined, lot-to-lot consistent material for in vitro and in vivo studies.
Specifications
| Isotype | Mouse IgG1, κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb mouse IgG1 isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
| Immunogen | Human T-cell acute lymphoblastic leukemia lines CEM and HSB-2 |
| Reported Applications |
in vivo CD7 blockade* in vivo therapy (CD7⁺ tumor cell elimination) * in vivo radioimmunodetection* in vitro complement-dependent cytotoxicity* *Baum et al., Br J Haematol 1996 · PMID 8904888 Flow cytometry* Immunofluorescence* Epitope competition (scFvCD7-GFP)* in vitro targeted cytotoxicity* *Peipp et al., Cancer Res 2002 · PMID 12019163 |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from mammalian cell supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |