Catalog #SIMB0099
InVivoSIM bispecific anti-human PD-L1 x VEGF-A (Pumitamig Biosimilar)
Clone
Pumitamig
Reactivities
Human
Isotype
Human IgG1, κ
Product Description
This Pumitamig biosimilar antibody uses the same variable regions as the therapeutic antibody Pumitamig (formerly BNT327), making it ideal for research use. Pumitamig is a bispecific IgG1 antibody that simultaneously targets human PD-L1 and VEGF-A, integrating immune checkpoint blockade with anti-angiogenic activity in a single molecule. By binding PD-L1, Pumitamig disrupts PD-1/PD-L1–mediated inhibitory signaling and restores T-cell activation within the tumor microenvironment. Concurrently, binding to VEGF-A inhibits VEGF signaling, reducing tumor angiogenesis, vascular permeability, and VEGF-driven immunosuppression. This dual-targeting strategy is designed to enhance antitumor immune responses by improving immune cell infiltration, normalizing tumor vasculature, and counteracting VEGF-associated resistance mechanisms to checkpoint inhibition. Pumitamig has shown promising clinical activity across multiple solid tumor indications, particularly in immunologically “cold” tumors where combined angiogenesis inhibition and immune activation may be advantageous. This Pumitamig biosimilar is well suited for studying PD-L1 biology, VEGF-mediated angiogenesis, immune–vascular crosstalk, tumor microenvironment remodeling, and for use as a research tool to benchmark the therapeutic antibody’s mechanism of action in pre-clinical experimental systems.
Specifications
| Isotype | Human IgG1, κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Reported Applications |
in vivo functional assays in vitro functional assays ELISA |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |