Catalog #SIMB0107
InVivoSIM bispecific anti-human PD-1 x VEGF-A (Ivonescimab Biosimilar)
Clone
Ivonescimab
Reactivities
Human
Applications
in vivo functional assays
in vitro functional assays
ELISA
in vitro functional assays
ELISA
Isotype
Human IgG1, κ , ScFv
Product Description
This Ivonescimab biosimilar antibody uses the same variable regions as the therapeutic antibody Ivonescimab, making it ideal for research use. Ivonescimab is a humanized, tetravalent bispecific IgG1-based antibody that simultaneously targets human PD-1 and VEGF-A. Its symmetric IgG-scFv architecture consists of an anti-VEGF-A IgG backbone with anti-PD-1 single-chain variable fragments (scFvs) fused to the C-termini of the heavy chains, providing two VEGF-A- and two PD-1-binding sites. By simultaneously blocking PD-1 checkpoint signaling and neutralizing VEGF-A, Ivonescimab combines immune checkpoint inhibition with suppression of VEGF-mediated angiogenesis and immunosuppression. The tetravalent architecture is designed to promote cooperative target engagement within the tumor microenvironment, where VEGF-A concentrations are elevated, potentially enhancing PD-1 blockade at sites of disease. This Ivonescimab biosimilar is well suited for studying PD-1 biology, VEGF-mediated angiogenesis, immune–vascular crosstalk, tumor microenvironment remodeling, dual checkpoint/anti-angiogenic strategies, and for benchmarking the therapeutic antibody's mechanism of action and activity in pre-clinical experimental systems.
Specifications
| Isotype | Human IgG1, κ , ScFv |
|---|---|
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | L234A/L235A |
| Reported Applications |
in vivo functional assays in vitro functional assays ELISA |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |