Catalog #SIMB0094
InVivoSIM bispecific anti-human CD3 x CD20 (Epcoritamab Biosimilar)
Clone
Epcoritamab
Reactivities
Human
Applications
in vivo functional assays
in vitro functional assays
ELISA
in vitro functional assays
ELISA
Isotype
Human IgG1
Product Description
This Epcoritamab biosimilar antibody uses the same variable regions as the therapeutic antibody Epcoritamab, making it ideal for research use. Epcoritamab is a full-length bispecific IgG1 antibody that simultaneously targets human CD3 on T cells and human CD20 on B cells. By engaging CD3 and CD20, Epcoritamab redirects and activates T cells against malignant B cells, promoting synapse formation, T-cell activation, cytokine release, and targeted tumor-cell killing. Unlike earlier bispecific formats, this IgG-based architecture provides extended half-life, efficient T-cell engagement, and potent in vivo activity. Clinically, Epcoritamab has demonstrated significant efficacy in relapsed or refractory B-cell non-Hodgkin lymphomas, including diffuse large B-cell lymphoma (DLBCL), and works through direct T-cell–mediated cytotoxicity rather than Fc-dependent mechanisms. This Epcoritamab biosimilar is well suited for investigating T-cell redirection, CD3/CD20 synapse biology, B-cell malignancies, immune-cell cytotoxicity, and the mechanistic basis of bispecific antibody therapies.
Specifications
| Isotype | Human IgG1 |
|---|---|
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | K214R/L234F/L235E/D265A |
| Reported Applications |
in vivo functional assays in vitro functional assays ELISA |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| RRID | AB_3751727 |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |