InVivoSIM anti-respiratory syncytial virus (RSV) F protein (Nirsevimab Biosimilar)
Catalog #SIM0088
Clone:
Nirsevimab
Reactivities:
Human
Product Details
This non-therapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody Nirsevimab, making it ideal for research use. Nirsevimab is a human IgG1 monoclonal antibody that targets the fusion (F) glycoprotein of respiratory syncytial virus (RSV), a highly contagious virus that causes serious respiratory infections, particularly in infants and older adults. The RSV F protein plays a critical role in viral entry by mediating the fusion of viral and host cell membranes, making it a key target for neutralization. Nirsevimab binds to a highly conserved epitope on the prefusion conformation of the RSV F protein, thereby preventing the conformational changes required for membrane fusion and subsequent infection. Unlike palivizumab, which binds both pre- and postfusion forms, Nirsevimab is specific to the prefusion form, enabling higher potency and prolonged protection. This Nirsevimab biosimilar is ideal for studying RSV entry mechanisms, neutralizing antibody responses, and RSV vaccine or therapeutic development.Specifications
| Isotype | Human IgG1, Īŗ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | K214R/M252Y/S254T/T256E |
| Immunogen | RSV F protein |
| Reported Applications |
In vivo functional assays in vitro functional assays Flow cytometry ELISA |
| Formulation |
pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<0.5EU/mg (<0.0005EU/Ī¼g) Determined by LAL gel clotting assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 Āµm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theilerās Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
Recommended Products
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Recommended Isotype Control(s)
RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer