Catalog #SIM0123FAB
InVivoSIM anti-human VEGF-A (Ranibizumab Biosimilar)
Clone
Ranibizumab
Reactivities
Human
Applications
In vivo functional assays
in vitro functional assays
in vitro functional assays
Isotype
Human IgG1, κ
Product Description
This Ranibizumab biosimilar antibody uses the same variable regions as the therapeutic antibody Ranibizumab, making it ideal for research use. Ranibizumab is a humanized antigen-binding fragment (Fab) that specifically targets human vascular endothelial growth factor A (VEGF-A), a key regulator of angiogenesis and vascular permeability. Unlike full-length IgG antibodies, Ranibizumab consists solely of the Fab region and therefore lacks an Fc domain and Fc-mediated effector functions. By binding soluble VEGF-A, Ranibizumab prevents VEGF-A from activating its receptors, including VEGFR-1 and VEGFR-2, thereby inhibiting VEGF-mediated endothelial cell proliferation, vascular permeability, and neovascularization. VEGF-A plays important roles in physiological and pathological angiogenesis and is particularly relevant to ocular neovascular diseases and tumor vascular biology. This Ranibizumab biosimilar is well suited for studying VEGF-A biology, angiogenesis, vascular permeability, endothelial cell signaling, ocular neovascularization, and for benchmarking the therapeutic antibody's target engagement and neutralizing activity in pre-clinical experimental systems.
Specifications
| Isotype | Human IgG1, κ |
|---|---|
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | Human VEGF-A |
| Reported Applications |
In vivo functional assays in vitro functional assays |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE and SEC |
| Sterility | 0.2 µm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| Molecular Weight | 48 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |